Regulatory

Part:BBa_K2207010

Designed by: Shisheng Li   Group: iGEM17_ZJU-China   (2017-10-23)


phlo Promoter

BBa_K2207010

Promoter(phlF regulated)

This part is an inducible promoter regulated by PhlF. And the sequence of it is offered by Jef D.Boeke's laboratory.

It is a modified ADH1 promoter from the yeast Saccharomyces cerevisiaea having a phlF operator sequence (phlO) embedded downstream the promoter. The phlO region can be bound by phlF, a regulatory protein existing in Pseudomonas fluorescens and related species.

In the absence of PhlF protein, this part promotes transcription.

In the presence of PhlF protein, this part inhibits transcription.

PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG).

PhlF can be coded by BBa_K2207009.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
regulator
Parameters
None